DNA Extraction and Purification
Takara Bio NucleoBond Xtra Maxi EF, 50 preps
NucleoBond Xtra Maxi EF 50 preps for the isolation of endotoxin-free plasmid DNA - NucleoBond Xtra Maxi Columns, NucleoBond Xtra Maxi Column Filters, buffers, RNase A.
Zymo Research Corporation Quick-DNA Fungal/Bacterial 96 Kit (2 x 96 Preps)
The Quick-DNA Fungal/Bacterial 96 Kit is designed for the simple and rapid isolation of DNA from tough-to-lyse fungi, including A. fumigatus, C. albicans, N. crassa, S. cerevisiae, S. pombe, as well as Gram (+/-) bacteria, algae, and protozoa. The procedure is easy and can be completed in minutes: fungal and/or bacterial samples are rapidly and efficiently lysed with our state of the art, ultra-high density BashingBeads. Zymo-Spin column technology is then used to isolate the DNA that is ideal for downstream molecular-based applications including PCR, array, etc.
Biochain Institute Inc DNA Methylation Detection Kit, 1 kit/PK
BioChain's DNA methylation detection kit offers a fast, efficient and convenient alternative to traditional bisulfite conversions. Our kit provides users with a true endogenous human positive control which eliminates any ambiguity in validating results. The positive control has been tested in over 15 different human tissues. We have optimized our protocol to minimize DNA degradation, reagent preparation, and conversion time. Our protocol guarantees > 99 % conversion of cytosine to uracil and > 99 % CpG protection, as calculated by sequencing, and is sensitive over a range of 2 ug - 500 pg of DNA. In less than 3 hours, users can obtain high quality bisulfite modified DNA ready for analysis by PCR, sequencing, or microarrays.
Biochain Institute Inc Genomic DNA - Rat Normal Tissue: Thymus, 100 ug/PK
BioChain's tissue genomic DNAs are isolated from a wide variety of tissues using a proprietary modified guanidine thiocyanate technique. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0, and A260/230 is >2.0 (detected in 10 mM Tris-Cl, pH 7.5). Contamination of RNA, polysaccharides and proteoglycans has been effectively eliminated.
Biochain Institute Inc CancerSeq Paraffin Tissue Tumor Block: Colon, 1 Block/pk
To generate our CancerSeq line of tissues, genomic DNA was extracted from the FFPE tissues and validated by targeted NGS with Illumina's TruSeq Amplicon - Cancer Panel (TSACP), which includes 48 cancer-related genes. Our prescreened tissues come with detailed information regarding donor and cancer type, as well as NGS information such as chromosomal position, variant type (SNP or indel), alternate allele variations, quality score, depth of coverage, allele type, transcript ID and more. We offer the prescreened tissue as mounted sections or whole block. Please inquire for other options, such as curls or extracted nucleic acids.
PACIFIC BIOSCIENCES OF CALIFORNIA INC Pacific Biosciences Paramagnetic Beads, SMRT Sequencing Workflow, Research Use Only
AMPure PB contains specially formulated paramagnetic beads for the SMRT™ sequencing workflow. This liquid suspension, appearing as brown beads in a clear liquid, is intended for research use only.
- Specially formulated paramagnetic beads for SMRT™ sequencing workflow.
- Appearance is brown beads suspended in clear liquid.
- Beads may settle to the bottom of the bottle.
- No odor.
- pH of 8.0 to 8.4.
- Relative density of ˜1.127.
- Fully miscible in water.
- Contains Sodium azide < 0.1%.
- Shipped at ambient temperature (15°C to 30°C) and stored refrigerated (2°C to 8°C) upon receipt.
- Mix reagent well before use to ensure homogenous and consistent color.
- Intended for research use only, not for diagnostic procedures.
Biochain Institute Inc Genomic DNA - Lupus: Kidney, from a single donor, 50 ug/PK
BioChain's tissue genomic DNAs are isolated from a wide variety of tissues using a proprietary modified guanidine thiocyanate technique. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0, and A260/230 is >2.0 (detected in 10 mM Tris-Cl, pH 7.5). Contamination of RNA, polysaccharides and proteoglycans has been effectively eliminated.
Zymo Research Corporation Quick-DNA Fungal/Bacterial Midiprep Kit (25 Preps)
The procedure is easy and can be completed in as little as 20 minutes: fungal and/or bacterial samples are added directly to a ZR BashingBead™ Lysis/Filtration Tube and rapidly and efficiently lysed by bead beating without using organic denaturants or proteinases. The DNA is isolated and purified using our FastSpin column technology and is ideal for downstream molecular-based applications including PCR, array, etc.
Zymo Research Corporation Zymoclean™ Gel DNA Recovery Kit (50 Preps) w/ Zymo-Spin™ IC Columns (Capped)
Simply add the specially formulated Agarose Dissolving Buffer (ADB) to the gel slice containing your DNA sample, let dissolve, and then transfer to the suppliedZymo-Spin™ Column. There is no need for organic denaturants or chloroform. Instead, the product utilizes Fast-Spin column technology to yield high-quality DNA in just 15 minutes). DNA purified using the Zymoclean™ Gel DNA Recovery Kit is perfectly suited for use in DNA ligation reactions, sequencing, DNA labeling reactions, PCR, etc.
New England Biolabs, Inc. NEBNext Ultra II FS DNA PCR-free Library Prep Kit for Illumina - 24 rxn
The NEBNext Ultra II DNA PCR-free Library Prep Kit contains the enzymes and buffers required to convert a broad range of intact DNA input amounts into libraries for next-generation sequencing on the Illumina platform all without an amplification step. The fast user-friendly workflow includes enzymatic fragmentation for optimal input size requires minimal hands-on time and maintains excellent library yields and GC coverage without requiring PCR. Dont be biased The NEBNext Ultra II FS DNA PCR-free Library Prep Kit for Illumina can save you time and minimize amplification-induced sequence bias and errors with a complete solution for PCR-free NGS library preparation. Built on the robust and reliable Ultra II reagents and workflow from New England Biolabs you can trust that your samples are in the best hands your own.
Boca Scientific Inc Buccal-Prep Plus DNA Isolation Kit, 50 Extractions
The Buccal-Prep Plus DNA Isolation Kit is a precipitation-based kit combining all the necessary optimized buffers to fully lyse and simply purify DNA from buccal swabs. The kit has a fast protocol that produces high yields and purity for all downstream processing applications. As with our other precipitation-based chemistries, no solvents, alcohol, or other external buffers are required, giving fewer processing steps and creating a faster process time.
Biochain Institute Inc Control Genomic DNA - Rabbit Female, 100 ug/PK
BioChain's tissue genomic DNAs are isolated from a wide variety of tissues using a proprietary modified guanidine thiocyanate technique. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0, and A260/230 is >2.0 (detected in 10 mM Tris-Cl, pH 7.5). Contamination of RNA, polysaccharides and proteoglycans has been effectively eliminated.
Biochain Institute Inc SeqPure PCR Purification Kit (5 ml), 5 ml/pk
SeqPure PCR reagent is a post PCR and Next Gen library prep clean up system based on paramagnetic beads technology, designed for an efficient purification of PCR amplicons. The purification consists of removal of salts, primers, primer-dimers, dNTPs, as DNA fragments are selectively bound to the magnetic beads particles; and highly purified DNA is eluted with low salt elution buffer or water and can be used directly for downstream applications. The protocol can be adapted to your current liquid handling workstation (e.g. Beckman, Hamilton, Tecan, Caliper, Perkin Elmer, Agilent and Eppendorf) utilizing your current protocol as well as it can be performed manually.